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The original thread on negative staining bacteriophages was in response to a query I made. I am appending to this message a slightly condensed summary of all the replies.
The Am Molybdate was different and needs further examination. I plan to try additional suggestions as soon as I get a fresh phage prep.
The advice once again proved how useful this listserver is to me. Thanks to everybody who shared their experiences. This made the Bacteria show up with good inner structure instead of "the blob". Perhaps a more dilute solution may stain a little less, but show up fine detail better?
If that doesn't work Maybe the fine details are embedded in a thick salt layer so that you don't get any contrast there. The procedure I normally use is: My experience is that usually it should work as nicely as UFo, but I was told that UAc does 'melt' more strongly and more quickly in the electron beam.
I am looking forward to other recipies.
I am very sure that you can find as many recipies as there are microscopists By the way, I have never been successful using PTA. Even with very sensitive proteins which don't like a low pH, I always had the best results in using Uranyl solutions.
My experience with UA is that when it works it's great but it doesn't always work.
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With PTA a bad batch of PTA stock could result in poor images - especially if it's old or not really a good quality reagent. Do you adjust the pH of your PTA? It is normally fairly acid and should be adjusted to between 6 and 7 but you can experiment.
We have always understood that it is best to use potassium hydroxide and not sodium hydroxide to adjust - personally I've seen little difference. If you are using carbon coatings on your grids and clean preparations of virus maybe you need a little BSA to help spreading not normally a problem with our samples.
If none of the above work it might be worth trying some of the newer more expensive stains like sodium silicotungstate or methylamine tungstate which can give nice results.
I suspect you may have tried most of the above, but I hope that something will help. This works well if you hold the grid at a 45 degree angle. If you wash try 0.
Water can osmotically shock the specimen.attheheels.com is a platform for academics to share research papers. From: Bastien Nocera ; To: commits-list gnome org; Cc: ; Subject: [totem-pl-parser] lib: Fix some RSS feeds using the "guid" field; Date: Thu, 26 Jul + (UTC).
Dear all, We are looking for a way to set the contrast and the brightness values in the SEM's backscatter mode at the same level from day to day and rather so.
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